Step 1:
At the top of the screenshot editor window, click the Highlighter button.
Step 2:
On the right, click Add Highlight to create a highlight rectangle on the image.
Step 3:
Set the color and opacity of the selected highlight using the options on the right.
Step 4:
Move and resize the highlight on the image:
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Drag by the green arrow circle 
or the white circles on the edges
to resize the highlight.
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Drag the edge of the highlight rectangle to move it.
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To rotate the highlight, drag the green circle left or right: 
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To delete the highlight, click the green circle with the cross
.